THE CYANOBACTERIUM, SYNECHOCOCCUS SP PCC7942, POSSESSES 2 DISTINCT GENES ENCODING CATION-TRANSPORTING P-TYPE ATPASES

被引：37

作者：

KANAMARU, K ^{[1
]}

KASHIWAGI, S ^{[1
]}

MIZUNO, T ^{[1
]}

机构：

[1] NAGOYA UNIV, SCH AGR, MOLEC MICROBIOL LAB, CHIKUSA KU, NAGOYA, AICHI 464, JAPAN

来源：

FEBS LETTERS | 1993年 / 330卷 / 01期

关键词：

CATION TRANSPORT; P-TYPE ATPASE; CYANOBACTERIUM; OSMOTIC ADAPTATION;

D O I：

10.1016/0014-5793(93)80928-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

P-type (or E1 E2-type) ATPases comprise a large family of prokaryotic and eukaryotic proteins capable of transporting a variety of cations. and function in a wide variety of cellular processes. The present study was carried out to search for genes encoding P-type ATPases in the phototrophic cyanobacterium, Synechococcus sp. PCC7942. We succeeded in cloning two genes each encoding P-type ATPases from this bacterium. It was found that Synechococcus at least, two distinct P-type ATPases; one belongs to the family of typical prokaryotic P-type ATPases and the other markedly resembles eukaryotic P-type ATPases. An insertion mutant lacking either of these two ATPase-genes was constructed. The results showed that the growth of these mutants is hypersensitive to osmotic stress upon addition of NaCl or sorbitol to the medium.

引用

页码：99 / 104

页数：6

共 24 条

[1] IONIC OSMOREGULATION DURING SALT ADAPTATION OF THE CYANOBACTERIUM SYNECHOCOCCUS 6311 [J].