METHYLENETETRAHYDROFOLATE-MEDIATED BIOSYNTHESIS OF RIBOTHYMIDINE IN THE TRANSFER-RNA OF STREPTOCOCCUS-FAECALIS - INCORPORATION OF HYDROGEN FROM SOLVENT INTO THE METHYL MOIETY

被引：14

作者：

DELK, AS ^{[1
]}

NAGLE, DP ^{[1
]}

RABINOWITZ, JC ^{[1
]}

STRAUB, KM ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,SPACE SCI LAB,BERKELEY,CA 94720

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)90858-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The methyl carbon of ribothymidine in the tRNA of Streptococcus faecalis is derived from 5,10-methylenetetrahydrofolate, not S-adenosylmethionine. Isotope labeling experiments have shown that the reduction of the methylene carbon of the folate cofactor to the methyl carbon of the modified residue involves a mechanism in which hydrogen from solvent is incorporated into the methyl moiety. Although the identity of the reducing agent involved directly in this novel methylation remains to be established, data suggest that reduced flavin serves this function in vitro. © 1979.

引用

页码：244 / 251

页数：8

共 12 条

[1] OCCURRENCE AND BIOSYNTHESIS OF RIBOTHYMIDINE IN TRANSFER-RNAS OF B-SUBTILIS [J].