DAMAGE TO LYMPHOCYTES BY X-RAY AND BLEOMYCIN MEASURED WITH THE CYTOKINESIS-BLOCK MICRONUCLEUS TECHNIQUE

被引:21
作者
ODAGIRI, Y
DEMPSEY, JL
MORLEY, AA
机构
[1] Department of Haematology, Flinders Medical Center, Bedford Park
来源
MUTATION RESEARCH | 1990年 / 237卷 / 3-4期
关键词
(Aging); Belomycin; Lymphocytes; Micronuclei; X-rays;
D O I
10.1016/0921-8734(90)90020-R
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Chromosome damge induced by X-irradiation or bleomycin was measured using the cytokinesis-block micronucleus assay in the peripheral blood lymphocytes of 6 newborn, 8 young and 10 elderly individuals. An increase in the frequecy of spontaneous micronuclei with age was observed. There was no difference in the X-irradiation-induced micronucleus frequency between the 3 groups. There was a significant increase with age in the number of micronuclei induced by bleomycin. Kinetochore-labelling studies revealed that the percentage of kinetochorone-positive induced micronuclei was higher for bleomycin (36.2-43.3%) that for X-irradiation (17.1-19.7%). The age-related increase in frequency of spontaneous or bleomycin-induced micronuclei was due to increases in both kinetochrone-positive and kinetochrone-negative micronuclei. The frequency of kinetochrone-positive or -negative micronuclei induced by X-irradiation was not different between the 3 age groups. There results suggest that bleomycin is more potent in inducing whole-chromsome loss than X-rays and that lymphocytes from aged individuals are more sensitive to bleomycin in terms of both chromosome breakage and whole chromosome loss. © 1990.
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页码:147 / 152
页数:6
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