DOXORUBICIN ENCAPSULATED IN STERICALLY STABILIZED LIPOSOMES FOR THE TREATMENT OF A BRAIN-TUMOR MODEL - BIODISTRIBUTION AND THERAPEUTIC EFFICACY

被引:198
作者
SIEGAL, T [1 ]
HOROWITZ, A [1 ]
GABIZON, A [1 ]
机构
[1] SHARETT INST ONCOL,JERUSALEM,ISRAEL
关键词
BRAIN NEOPLASM; CEREBROSPINAL FLUID; DOXORUBICIN; LIPOSOME PHARMACOKINETICS;
D O I
10.3171/jns.1995.83.6.1029
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Anthracyclines entrapped in small-sized, sterically stabilized liposomes have the advantage of long circulation time, reduced systemic toxicity, increased uptake into systemic tumors, and gradual release of their payload. To date, there is no information on the behavior of these liposomes in brain tumors. The objective of this study was to compare the biodistribution and clinical efficacy of free doxorubicin (F-DOX) and stealth liposome-encapsulated DOX (SL-DOX) in a secondary brain tumor model. Nine days after tumor inoculation Fischer rats with a right parietal malignant sarcoma received an intravenous dose of 6 mg/kg of either F-DOX or SL-DOX for evaluation of drug biodistribution. For therapeutic trials a single dose of 8 mg/kg was given 6 or 11 days after tumor induction, or alternatively, weekly doses (5 mg/kg) were given on Days 6, 13, and 20. Liposome-encapsulated DOX was slowly cleared from plasma with a t(1/2) of 35 hours. Free-DOX maximum tumor drug levels reached a mean value of 0.8 mu g/g and were identical in the adjacent brain and contralateral hemisphere. In contrast, SL-DOX tumor levels were 14-fold higher at their peak levels at 48 hours, declining to ninefold increased levels at 120 hours. A gradual increase in drug levels in the brain adjacent to tumor was noted between 72 and 120 hours (up to 4 mu g/g). High-performance liquid chromatography analysis identified a small amount of aglycone metabolites within the tumor mass from 96 hours and beyond, after SL-DOX injection. Cerebrospinal fluid levels were barely detectable in tumor-bearing rats treated with F-DOX up to 120 hours after drug injection (less than or equal to 0.05 mu g/ml), whereas the levels found after SL-DOX were 10- to 30-fold higher. An F-DOX single-dose treatment given 6 days after tumor inoculation increased the rats' life span (ELS) by 135% over controls (p < 0.05) but was not effective if given on Day 11. In contrast, SL-DOX treatment resulted in an]LS of 168% (p < 0.0003) with no difference when given after 6 or 11 days. Treatment with three weekly doses of SL-DOX produced an ILS of 189% compared to 126% by F-DOX (p < 0.0002). The authors conclude that the use of long-circulating liposomes as cytotoxic drug carriers in brain tumor results in enhanced drug exposure and improved therapeutic activity, with equal effectiveness against early small- and large-sized brain tumors.
引用
收藏
页码:1029 / 1037
页数:9
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