TRANSFORMING GROWTH-FACTOR-BETA-1 INDUCES APOPTOTIC CELL-DEATH IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS WITH DOWN-REGULATED EXPRESSION OF BCL-2

被引：104

作者：

TSUKADA, T ^{[1
]}

EGUCHI, K ^{[1
]}

MIGITA, K ^{[1
]}

KAWABE, Y ^{[1
]}

KAWAKAMI, A ^{[1
]}

MATSUOKA, N ^{[1
]}

TAKASHIMA, H ^{[1
]}

MIZOKAMI, A ^{[1
]}

NAGATAKI, S ^{[1
]}

机构：

[1] NAGASAKI UNIV,SCH MED,DEPT INTERNAL MED 1,NAGASAKI 852,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1766

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor beta 1 (TGF-beta 1) is a potent growth inhibitor of human umbilical vein endothelial cells (HUVEC). We investigated whether apoptosis is involved in the TGF-beta 1 mediated HUVEC growth inhibition. Using immunofluorescence microscopy and staining by propidium iodide, we showed HUVEC treated with TGF-beta 1 contained characteristic apoptotic nuclei with condensation of chromatin. FAGS analysis demonstrated that the number of cells with hypodiploid DNA contents were increased in HUVEC treated with TGF-beta 1. The DNA fragmentation assay exhibited typical ladder formation in TGF-beta 1-treated HUVEC. Using Western blot analysis, the expression of bcl-2 on HUVEC was down-regulated by TGF-beta 1-treatment. These findings suggest that TGF-P1 regulates the growth of HUVEC by inducing apoptosis accompanied with down-regulation of bcl-2 expression. (C) 1995 Academic Press, Inc.

引用

页码：1076 / 1082

页数：7

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