A DISSECTION OF THE CAULIFLOWER MOSAIC-VIRUS POLYADENYLATION SIGNAL

被引：98

作者：

SANFACON, H ^{[1
]}

BRODMANN, P ^{[1
]}

HOHN, T ^{[1
]}

机构：

[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND

来源：

GENES & DEVELOPMENT | 1991年 / 5卷 / 01期

关键词：

MESSENGER RNA 3'-END FORMATION; PLANT POLYADENYLATION SIGNAL; CAULIFLOWER MOSAIC VIRUS; AATAAA MUTATION; UPSTREAM DOWNSTREAM DELETIONS; RNASE PROTECTION ASSAY;

D O I：

10.1101/gad.5.1.141

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mutagenesis analysis of the polyadenylation [poly(A)] signal from the cauliflower mosaic virus (CaMV), a plant pararetrovirus, revealed striking differences to known vertebrate poly(A) signals. Our results show that (1) the AATAAA sequence is necessary for efficient cleavage at the poly(A) site, although the requirement for an authentic AATAAA might be less stringent in plant than in vertebrate cells; (2) surprisingly and in contrast to the majority of vertebrate poly(A) signals, the sequences downstream the CaMV poly(A) site do not influence processing efficiency drastically although they affect the precision of cleavage; and (3) deletion of sequences upstream of the CaMV AATAAA sequence decreased processing at the CaMV site dramatically, suggesting the presence of one or several positively acting upstream elements. An oligonucleotide consisting of CaMV upstream sequences could induce the recognition of a normally silent exogenous poly(A) signal when inserted upstream of its AATAAA motif.

引用

页码：141 / 149

页数：9

共 62 条

[1] FUNCTIONAL-ANALYSIS OF THE 3' CONTROL REGION OF THE POTATO WOUND-INDUCIBLE PROTEINASE INHIBITOR-II GENE [J].