FRACTIONATION OF RABBIT ANTI-HORSE CYTOCHROME-C .1. SPECIFICITY OF THE ISOLATED FRACTIONS

被引：21

作者：

ENG, J ^{[1
]}

REICHLIN, M ^{[1
]}

机构：

[1] VET ADM HOSP,BUFFALO,NY 14215

来源：

MOLECULAR IMMUNOLOGY | 1979年 / 16卷 / 04期

关键词：

D O I：

10.1016/0161-5890(79)90060-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been devised to fractionate rabbit antibodies to horse cytochrome c by affinity chromatography. Immune serum was passed through a series of affinity columns (immunoadsorbents) each prepared with cytochromes c from closely related species. Antibody sub-populations were isolated by selection of the proper sequence of columns, and displayed unitary stoichiometry of reaction (one Fab' molecule bound per cytochrome c molecule) in fluorescence quenching experiments. The localization of the antigenic determinants towards which these tractions were directed was deduced from the pattern of the cross-reactions of such fractions with cytochromes c of known amino acid sequence. Analysis of these fractions reveals that residues 44 (proline). 60 (lysine) and 92 (glutamic acid) influence the structure of individual antigenic determinants of horse cytochrome c. © 1979.

引用

页码：225 / 230

页数：6

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