AMMONIA RHYTHM IN MICROCYSTIS-FIRMA STUDIED BY INVIVO N-15 AND P-31 NMR-SPECTROSCOPY

Cultures of the cyanobacterium Microcystis firma show rhythmic uptake and release of ammonia Linder conditions of carbon limitation. The massive removal of ammonia from the medium during the first light phase has little impact on the intracellular pH: a pH shift of less than 0.2 U towards the alkaline can be measured by in vivo P-31 NMR. Furthermore, the energy status of the cells remains regulated. In vivo N-15 NMR of M. firma, cultivated either with labelled nitrate or ammonia as the sole nitrogen source, reveals only gradual differences in the pool of free amino acids. Additionally both cultivation types show gamma-aminobutyric acid, acid amides and yet unassigned secondary metabolites as nitrogen storing compounds. Investigating the incorporation of nitrogen under carbon limitation, however, only the amide nitrogen of glutamine is found permanently labelled in situ. While transamination reactions are blocked, nitrate reduction to ammonia can still proceed. Cation exchange processes in the cell wall are considered regarding the ammonia disappearance in the first phase, and the control of ammonia uptake is discussed with respect to the avoidance of intracellular toxification.