MEMBRANE-FRACTION COLLECTION FOR CAPILLARY ELECTROPHORESIS

被引：31

作者：

CHENG, YF

FUCHS, M

ANDREWS, D

CARSON, W

机构：

[1] Millipore Corporation, Waters Chromatography Division, Milford, MA 01757

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 608卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)87112-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple instrument system combining high-performance capillary electrophoresis (CE) and membrane technology is described. CE fraction collection is successfully implemented using a membrane assembly at the exit end of a capillary to complete the electrical circuit for electrophoretic separation. This membrane assembly consists of a poly(vinylidene difluoride) membrane, a buffer reservoir (two layers of 3 MM Chrom filter-paper) and a stainless-steel plate as the ground electrode. Two model proteins are separated and collected on the membrane. Direct protein sequencing is demonstrated from this membrane after CE fraction collection.

引用

页码：109 / 116

页数：8

共 20 条

[1]

AEBERSOLD RH, 1986, J BIOL CHEM, V261, P4229

[2]

BECK S, 1986, ELECTORPHORESIS 86, P173

[3] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE [J].