D-L AMINO-ACID-ANALYSIS USING AUTOMATED PRECOLUMN DERIVATIZATION WITH 1-FLUORO-2,4-DINITROPHENYL-5-ALANINE AMIDE

被引：14

作者：

SCALONI, A

SIMMACO, M

BOSSA, F

机构：

[1] Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli', Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università 'La Sapienza', Roma, I-00185

来源：

AMINO ACIDS | 1995年 / 8卷 / 03期

关键词：

AMINO ACID ENANTIOMERIC MIXTURES; MARFEY REAGENT; EDMAN DEGRADATION; RP-HPLC;

D O I：

10.1007/BF00806826

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated procedure for the precolumn derivatization of enantiomeric amino acid mixtures with 1-fluoro-2,4-dinitrophenyl-5-alanine amide and a liquid chromatographic method for the separation of the derivatives with UV detection are reported. The system described allows to perform routine analyses using microbore columns with a sensitivity at the picomol level. Improvements for the use of this reagent in the protocol of a subtractive Edman degradation procedure of peptides to determine the sequence position of amino acid residues with concomitant identification of their chirality are also described.

引用

页码：305 / 313

页数：9

共 21 条

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