SHORT-TERM IN-VITRO CULTURE AND MOLECULAR ANALYSIS OF THE MICROSPORIDIAN, ENTEROCYTOZOON-BIENEUSI

被引:54
作者
VISVESVARA, GS
LEITCH, GJ
PIENIAZEK, NJ
DASILVA, AJ
WALLACE, S
SLEMENDA, SB
WEBER, R
SCHWARTZ, DA
GORELKIN, L
WILCOX, CM
BRYAN, RT
机构
[1] CTR DIS CONTROL & PREVENT,PUBL HLTH SERV,US DEPT HLTH & HUMAN SERV,NATL CTR INFECT DIS,ATLANTA,GA 30341
[2] MOREHOUSE SCH MED,ATLANTA,GA 30310
[3] EMORY UNIV,GRADY MEM HOSP,SCH MED,DEPT MED,ATLANTA,GA 30335
[4] EMORY UNIV,GRADY MEM HOSP,SCH MED,DEPT PATHOL,ATLANTA,GA 30335
关键词
AIDS-DIARRHEA; ELECTRON MICROSCOPY; ENTEROCYTOZOON BIENAUSI; ENTEROCYTES; MICROSPORIDIA; PCR; SEQUENCE ANALYSIS;
D O I
10.1111/j.1550-7408.1995.tb05896.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The microsporidium, Enterocytozoon bieneusi, causes a severe, debilitating, chronic diarrhea in patients with the acquired immunodeficiency syndrome. Specific diagnosis of intestinal microsporidiosis, especially due to Enterocytozoon, is difficult and there is no known therapy that can completely eradicate this parasite. Preliminary studies indicate that a short term (about 6 months) in vitro culture of this parasite yielding low numbers of spores, may be established by inoculating human lung fibroblasts and/or monkey kidney cell cultures with duodenal aspirates and or biopsy from infected patients. The cultures may subsequently be used for the isolation and molecular analysis of parasite DNA.
引用
收藏
页码:506 / 510
页数:5
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