IMMUNITY TO ST-LOUIS ENCEPHALITIS-VIRUS BY SEQUENTIAL IMMUNIZATION WITH RECOMBINANT VACCINIA AND BACULOVIRUS DERIVED PRM/E PROTEINS

St. Louis encephalitis (SLE) is an important mosquito-borne disease of great public health concern in parts of the United States, South America and Canada. Protective immunogens of flaviviruses produced in different expression systems have been shown to be effective against virulent virus infection in laboratory animal models. Here we show that the pre-membrane and envelope (PrM/E) of SLE virus expressed in insect and mammalian cell systems using baculovirus and vaccinia virus, respectively, are processed correctly and showed similar antigenic characteristics as the authentic proteins, Immunization with the recombinant proteins individually or in combination resulted in neutralizing and protective immune responses. A schedule consisting of initial immunization with recombinant vaccinia virus followed by a secondary boost with recombinant baculovirus protein resulted in higher levels of neutralizing and protective immune responses. The advantages of the use of such a combined approach as a general immunization strategy are discussed.