DISTINCT SPATIOTEMPORAL DISTRIBUTIONS OF THE N-METHYL-D-ASPARTATE RECEPTOR-CHANNEL SUBUNIT MESSENGER-RNAS IN THE MOUSE CERVICAL CORD

被引:112
作者
WATANABE, M [1 ]
MISHINA, M [1 ]
INOUE, Y [1 ]
机构
[1] NIIGATA UNIV, BRAIN RES INST, DEPT NEUROPHARMACOL, NIIGATA 951, JAPAN
关键词
GLUTAMATE RECEPTOR CHANNEL; SPINAL CORD; IN SITU HYBRIDIZATION; DEVELOPMENT;
D O I
10.1002/cne.903450212
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The distribution of five N-methyl-D-aspartate (NMDA) receptor channel subunit mRNAs in the mouse spinal cord from embryonic day 13 (E13) through postnatal day 56 (P56) was semiquantitatively examined at the cervical level via in situ hybridization with subunit-specific oligonucleotide probes. Signals for the epsilon 1 subunit mRNA were restricted to the most ventral portion of the spinal cord during embryonic stages. They extended to all laminae of the spinal cord except for the lamina 2 (substantia gelatinosa) during postnatal development. A wide expression of the epsilon 2 subunit mRNA was found in the spinal gray matter from E13 through neonatal stages, but the signals became restricted to the lamina 2 by P21. No significant signals for the epsilon 3 subunit mRNA were detected in the spinal cord at any developmental stages. The epsilon 4 subunit mRNA was distributed widely in the spinal cord during embryonic and early postnatal periods but decreased nearly to background levels by P21. In contrast to the differential distribution of the epsilon subunit mRNAs, the zeta 1 subunit mRNA was found ubiquitously at each developmental stage examined. These findings suggest that the molecular organization of the epsilon subunits may be different between the dorsal horn and the remaining regions in the mature spinal cord, which provides a molecular basis for functional heterogeneity of the NMDA receptor channel. Moreover, this spatial heterogeneity might be generated through drastic alterations in the subunit composition of the channel complex during spinal cord development. (C) 1994 Wiley-Liss, Inc.
引用
收藏
页码:314 / 319
页数:6
相关论文
共 40 条
[1]   CHANGES IN VOLTAGE DEPENDENCE OF NMDA CURRENTS DURING DEVELOPMENT [J].
BENARI, Y ;
CHERUBINI, E ;
KRNJEVIC, K .
NEUROSCIENCE LETTERS, 1988, 94 (1-2) :88-92
[2]   EVIDENCE FOR AN INVOLVEMENT OF NMDA AND NON-NMDA RECEPTORS IN SYNAPTIC EXCITATION OF PHRENIC MOTONEURONS IN THE RABBIT [J].
BOHMER, G ;
SCHMID, K ;
SCHAUER, W .
NEUROSCIENCE LETTERS, 1991, 130 (02) :271-274
[3]   ENHANCEMENT OF NMDA-EVOKED NEURONAL-ACTIVITY BY GLYCINE IN THE RAT SPINAL-CORD INVIVO [J].
BUDAI, D ;
WILCOX, GL ;
LARSON, AA .
NEUROSCIENCE LETTERS, 1992, 135 (02) :265-268
[4]  
CAUDLE RM, 1988, J PHARMACOL EXP THER, V246, P508
[5]   THE CHEMICAL EXCITATION OF SPINAL NEURONES BY CERTAIN ACIDIC AMINO ACIDS [J].
CURTIS, DR ;
PHILLIS, JW ;
WATKINS, JC .
JOURNAL OF PHYSIOLOGY-LONDON, 1960, 150 (03) :656-682
[6]  
DAVIES J, 1983, EXP BRAIN RES, V49, P280
[7]   REGION-SPECIFIC EXPRESSION OF SUBUNITS OF IONOTROPIC GLUTAMATE RECEPTORS (AMPA-TYPE, KA-TYPE AND NMDA RECEPTORS) IN THE RAT SPINAL-CORD WITH SPECIAL REFERENCE TO NOCICEPTION [J].
FURUYAMA, T ;
KIYAMA, H ;
SATO, K ;
PARK, HT ;
MAENO, H ;
TAKAGI, H ;
TOHYAMA, M .
MOLECULAR BRAIN RESEARCH, 1993, 18 (1-2) :141-151
[8]   DEVELOPMENTAL REGULATION OF NMDA RECEPTOR-MEDIATED SYNAPTIC CURRENTS AT A CENTRAL SYNAPSE [J].
HESTRIN, S .
NATURE, 1992, 357 (6380) :686-689
[9]   EXPERIMENTAL IMMUNOHISTOCHEMICAL STUDIES ON LOCALIZATION AND DISTRIBUTION OF SUBSTANCE-P IN CAT PRIMARY SENSORY NEURONS [J].
HOKFELT, T ;
KELLERTH, JO ;
NILSSON, G ;
PERNOW, B .
BRAIN RESEARCH, 1975, 100 (02) :235-252
[10]   CLONING AND EXPRESSION OF THE EPSILON-4 SUBUNIT OF THE NMDA RECEPTOR CHANNEL [J].
IKEDA, K ;
NAGASAWA, M ;
MORI, H ;
ARAKI, K ;
SAKIMURA, K ;
WATANABE, M ;
INOUE, Y ;
MISHINA, M .
FEBS LETTERS, 1992, 313 (01) :34-38