66 KILODALTON-RELATED ANTIGENS OF PLASMODIUM-KNOWLESI ARE MEROZOITE SURFACE-ANTIGENS ASSOCIATED WITH THE APICAL PROMINENCE

被引:22
作者
THOMAS, AW
BANNISTER, LH
WATERS, AP
机构
[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,DEPT ANAT,LONDON SE1 9RT,ENGLAND
[2] NIAID,MALARIA SECT,PARASIT DIS LAB,BETHESDA,MD 20892
关键词
66 kD antigen; immunoelectron microscopy; immunofluorescence; malaria; merozoite; Plasmodium knowlesi; red cell invasion; surface antigens;
D O I
10.1111/j.1365-3024.1990.tb00940.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Summary We have previously reported that Fab fragments of a monoclonal antibody to the 66 kD‐related antigens of Plasmodium knowlesi inhibit merozoite invasion of erythrocytes, strongly suggesting a role for these antigens as merozoite receptors for red cells. In this paper we have examined the distribution of these antigens on the surface of free merozoites. Rapid immunofluorescence microscopy on free, unfixed cells demonstrated a preferential association of antigen with a polar region of merozoites. This was confirmed, and localized essentially to the apical region, by immunoelectron microscopy under a variety of fixation regimes. Metabolic inhibitors did not affect the distribution, suggesting that apical localization is not due to capping induced by cross‐linking antibodies. These observations suggest that 66 kD‐related antigens may play a role in the orientation of the apical prominence of merozoites towards the red cell, and/or in the junction formation that occurs subsequent to orientation. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:105 / 113
页数:9
相关论文
共 14 条
[1]  
AIKAWA M, 1983, CIBA F SYMP, V94, P45
[2]  
BUTCHER GA, 1972, IMMUNOLOGY, V23, P503
[3]   A NATURALLY ACQUIRED QUOTIDIAN-TYPE MALARIA IN MAN TRANSFERABLE TO MONKEYS [J].
CHIN, W ;
CONTACOS, PG ;
COATNEY, GR ;
KIMBALL, HR .
SCIENCE, 1965, 149 (3686) :865-&
[4]   BIOSYNTHESIS OF A PUTATIVE PROTECTIVE PLASMODIUM-KNOWLESI MEROZOITE ANTIGEN [J].
DEANS, JA ;
THOMAS, AW ;
ALDERSON, T ;
COHEN, S .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1984, 11 (APR) :189-204
[5]  
DEANS JA, 1982, CLIN EXP IMMUNOL, V49, P297
[6]   VACCINATION TRIALS IN RHESUS-MONKEYS WITH A MINOR, INVARIANT, PLASMODIUM-KNOWLESI 66 KD MEROZOITE ANTIGEN [J].
DEANS, JA ;
KNIGHT, AM ;
JEAN, WC ;
WATERS, AP ;
COHEN, S ;
MITCHELL, GH .
PARASITE IMMUNOLOGY, 1988, 10 (05) :535-552
[7]   INVITRO ISOLATION OF PLASMODIUM-KNOWLESI MEROZOITES USING POLYCARBONATE SIEVES [J].
DENNIS, ED ;
MITCHELL, GH ;
BUTCHER, GA ;
COHEN, S .
PARASITOLOGY, 1975, 71 (DEC) :475-&
[8]   ANTIBODY-INDUCED MOVEMENT OF MEMBRANE COMPONENTS OF LEISHMANIA-ENRIETTII [J].
DOYLE, JJ ;
BEHIN, R ;
MAUEL, J ;
ROWE, DS .
JOURNAL OF EXPERIMENTAL MEDICINE, 1974, 139 (05) :1061-1069
[9]   PLASMODIUM-KNOWLESI - STUDIES ON INVASION OF RHESUS ERYTHROCYTES BY MEROZOITES IN THE PRESENCE OF PROTEASE INHIBITORS [J].
HADLEY, T ;
AIKAWA, M ;
MILLER, LH .
EXPERIMENTAL PARASITOLOGY, 1983, 55 (03) :306-311
[10]   ANTIGENICITY OF THE INFECTED ERYTHROCYTE AND MEROZOITE SURFACES IN FALCIPARUM-MALARIA [J].
LANGRETH, SG ;
REESE, RT .
JOURNAL OF EXPERIMENTAL MEDICINE, 1979, 150 (05) :1241-1254