STRUCTURE AND FUNCTION OF A BACTERIAL MESSENGER-RNA STABILIZER - ANALYSIS OF THE 5' UNTRANSLATED REGION OF OMPA MESSENGER-RNA

被引：95

作者：

CHEN, LH ^{[1
]}

EMORY, SA ^{[1
]}

BRICKER, AL ^{[1
]}

BOUVET, P ^{[1
]}

BELASCO, JG ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, DEPT MICROBIOL & MOLEC GENET, BOSTON, MA 02115 USA

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 15期

关键词：

D O I：

10.1128/jb.173.15.4578-4586.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 5' untranslated region (UTR) of the Escherichia coli ompA transcript functions in vivo as a growth rate-regulated mRNA stabilizer. The secondary structure of this mRNA segment has been determined by a combination of three methods: phylogenetic analysis, in vitro probing with a structure-specific RNase, and methylation by dimethylsulfate in vivo and in vitro. These studies reveal that despite extensive sequence differences, the 5' UTRs of the ompA transcripts of E. coli, Serratia marcescens, and Enterobacter aerogenes can fold in a remarkably similar fashion. Furthermore, the Serratia and Enterobacter ompA 5' UTRs function as effective mRNA stabilizers in E. coli. Stabilization of mRNA by the Serratia ompA 5' UTR is growth rate dependent. These findings indicate that the features of the ompA 5' UTR responsible for its ability to stabilize mRNA in a growth rate-regulated manner are to be found among the structural similarities shared by these diverse evolutionary variants.

引用

页码：4578 / 4586

页数：9

共 48 条

[1] INDUCED MESSENGER-RNA STABILITY IN BACILLUS-SUBTILIS [J].

BECHHOFER, DH ;

DUBNAU, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (02) :498-502

[2] THE STABILITY OF ESCHERICHIA-COLI GENE TRANSCRIPTS IS DEPENDENT ON DETERMINANTS LOCALIZED TO SPECIFIC MESSENGER-RNA SEGMENTS [J].

BELASCO, JG ;

NILSSON, G ;

VONGABAIN, A ;

COHEN, SN .

CELL, 1986, 46 (02) :245-251

[3] MECHANISMS OF MESSENGER-RNA DECAY IN BACTERIA - A PERSPECTIVE [J].

BELASCO, JG ;

HIGGINS, CF .

GENE, 1988, 72 (1-2) :15-23

[4] MOLECULAR CHARACTERIZATION OF THE GENE CODING FOR MAJOR OUTER-MEMBRANE PROTEIN OMPA FROM ENTEROBACTER-AEROGENES [J].

BRAUN, G ;

COLE, ST .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 137 (03) :495-500

[5] DNA-SEQUENCE ANALYSIS OF THE SERRATIA-MARCESCENS OMPA GENE - IMPLICATIONS FOR THE ORGANIZATION OF AN ENTEROBACTERIAL OUTER-MEMBRANE PROTEIN [J].

BRAUN, G ;

COLE, ST .

MOLECULAR & GENERAL GENETICS, 1984, 195 (1-2) :321-328

[6] MECHANISMS OF MESSENGER-RNA DECAY [J].

BRAWERMAN, G .

TRENDS IN BIOTECHNOLOGY, 1990, 8 (07) :171-174

[7] CLONED STRUCTURAL GENE (OMPA) FOR AN INTEGRAL OUTER-MEMBRANE PROTEIN OF ESCHERICHIA-COLI K-12 - LOCALIZATION ON HYBRID PLASMID PTU100 AND EXPRESSION OF A FRAGMENT OF THE GENE [J].

BREMER, E ;

BECK, E ;

HINDENNACH, I ;

SONNTAG, I ;

HENNING, U .

MOLECULAR AND GENERAL GENETICS, 1980, 179 (01) :13-20

[8] AN INTERCISTRONIC STEM-LOOP STRUCTURE FUNCTIONS AS AN MESSENGER-RNA DECAY TERMINATOR NECESSARY BUT INSUFFICIENT FOR PUF MESSENGER-RNA STABILITY [J].

CHEN, CYA ;

BEATTY, JT ;

COHEN, SN ;

BELASCO, JG .

CELL, 1988, 52 (04) :609-619

[9] POLYNUCLEOTIDE PHOSPHORYLASE AND RIBONUCLEASE-II ARE REQUIRED FOR CELL VIABILITY AND MESSENGER-RNA TURNOVER IN ESCHERICHIA-COLI K-12 [J].

DONOVAN, WP ;

KUSHNER, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (01) :120-124

[10] THE OMPA 5' UNTRANSLATED RNA SEGMENT FUNCTIONS IN ESCHERICHIA-COLI AS A GROWTH-RATE-REGULATED MESSENGER-RNA STABILIZER WHOSE ACTIVITY IS UNRELATED TO TRANSLATIONAL EFFICIENCY [J].

EMORY, SA ;

BELASCO, JG .

JOURNAL OF BACTERIOLOGY, 1990, 172 (08) :4472-4481

← 1 2 3 4 5 →