ISOLATION, CHARACTERIZATION, AND SEQUENCE OF AN ESCHERICHIA-COLI HEAT-SHOCK GENE, HTPX

被引：52

作者：

KORNITZER, D ^{[1
]}

TEFF, D ^{[1
]}

ALTUVIA, S ^{[1
]}

OPPENHEIM, AB ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MOLEC GENET,IL-91010 JERUSALEM,ISRAEL

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 09期

关键词：

D O I：

10.1128/JB.173.9.2944-2953.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We isolated and characterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a sigma-32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides.

引用

页码：2944 / 2953

页数：10

共 56 条

[1] ALTERNATIVE MESSENGER-RNA STRUCTURES OF THE CIII-GENE OF BACTERIOPHAGE-LAMBDA DETERMINE THE RATE OF ITS TRANSLATION INITIATION [J].

ALTUVIA, S ;

KORNITZER, D ;

TEFF, D ;

OPPENHEIM, AB .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 210 (02) :265-280

[2] TRANSLATIONAL REGULATORY SIGNALS WITHIN THE CODING REGION OF THE BACTERIOPHAGE -LAMBDA CIII GENE [J].

ALTUVIA, S ;

OPPENHEIM, AB .

JOURNAL OF BACTERIOLOGY, 1986, 167 (01) :415-419

[3] RNASE-III STIMULATES THE TRANSLATION OF THE CIII GENE OF BACTERIOPHAGE-LAMBDA [J].

ALTUVIA, S ;

LOCKERGILADI, H ;

KOBY, S ;

BENNUN, O ;

OPPENHEIM, AB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (18) :6511-6515

[4] ABNORMAL PROTEINS SERVE AS EUKARYOTIC STRESS SIGNALS AND TRIGGER THE ACTIVATION OF HEAT-SHOCK GENES [J].

ANANTHAN, J ;

GOLDBERG, AL ;

VOELLMY, R .

SCIENCE, 1986, 232 (4749) :522-524

[5] EUKARYOTIC MR 83,000 HEAT-SHOCK PROTEIN HAS A HOMOLOG IN ESCHERICHIA-COLI [J].

BARDWELL, JCA ;

CRAIG, EA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (15) :5177-5181

[6] MAJOR HEAT-SHOCK GENE OF DROSOPHILA AND THE ESCHERICHIA-COLI HEAT-INDUCIBLE DNAK GENE ARE HOMOLOGOUS [J].

BARDWELL, JCA ;

CRAIG, EA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (03) :848-852

[7] ANALYSIS OF PROCESSES OF INFECTION AND INDUCTION OF ESCHERICHIA-COLI MUTANT HFL-1 BY BACTERIOPHAGE-LAMBDA [J].

BELFORT, M ;

WULFF, DL .

VIROLOGY, 1973, 55 (01) :183-192

[8] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].

CHEN, EY ;

SEEBURG, PH .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170

[9] CLEAVAGE OF THE CII PROTEIN OF PHAGE-LAMBDA BY PURIFIED HFLA PROTEASE - CONTROL OF THE SWITCH BETWEEN LYSIS AND LYSOGENY [J].

CHENG, HH ;

MUHLRAD, PJ ;

HOYT, MA ;

ECHOLS, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7882-7886

[10] INTERACTION OF ESCHERICHIA-COLI RNA-POLYMERASE HOLOENZYME CONTAINING SIGMA-32 WITH HEAT-SHOCK PROMOTERS - DNASE-I FOOTPRINTING AND METHYLATION PROTECTION [J].

COWING, DW ;

GROSS, CA .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 210 (03) :513-520

← 1 2 3 4 5 6 →