LYSYL-TRANSFER RNA-SYNTHETASE GENE OF CAMPYLOBACTER-JEJUNI

被引:33
作者
CHAN, VL
BINGHAM, HL
机构
[1] Department of Microbiology, University of Toronto, Toronto
关键词
D O I
10.1128/jb.174.3.695-701.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We report the cloning and complete nucleotide sequence of the Campylobacter jejuni lysyl-tRNA synthetase gene (lysS). The C. jejuni lysS gene sequence shows high homology to the two Escherichia coli lysyl-tRNA synthetase genes, lysS and lysU. The Campylobacter lysyl-tRNA synthetase protein (LysRS) shows 47.9 and 46.6% sequence identity to the E. coli enzymes encoded by the lysS and lysU genes, respectively. The LysRS encoded by the C. jejuni gene is a polypeptide of 501 amino acids with a deduced molecular weight of 57,867. The enzyme is active in E. coli. The gene is expressed from its own promoter, and the transcription start site has been mapped. The carboxyl-terminal codon of the C. jejuni lysS gene overlaps by 1 bp with the Met initiation codon of the glyA gene, which has been shown to have a promoter which is functional in E. coli (V. L. Chan and H. L. Bingham, Gene 101:51-58, 1991). C. jejuni, unlike E. coli, has only one lysyl-tRNA synthetase gene.
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页码:695 / 701
页数:7
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