NITRIC-OXIDE SYNTHASE IN FERRET BRAIN - LOCALIZATION AND CHARACTERIZATION

被引:12
作者
MATSUMOTO, T
MITCHELL, JA
SCHMIDT, HHHW
KOHLHAAS, KL
WARNER, TD
FORSTERMANN, U
MURAD, F
机构
[1] Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois
[2] Abbott Laboratories, Illinois, 60064, Abbott Park
关键词
NITRIC OXIDE; NITRIC OXIDE SYNTHASE; ARGININE; CITRULLINE; GUANYLYL CYCLASE; CYCLIC GMP;
D O I
10.1111/j.1476-5381.1992.tb14535.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1 In the present study, we have investigated the distribution of nitric oxide synthase in the ferret brain. Nitric oxide snythase was determined biochemically and immunochemically. 2 In the rat brain, the highest nitric oxide snythase activity has been detected in the cerebellum. However, in the ferret brain, the highest activity was found in the striatum and the lowest in the cerebellum and cerebral cortex. The enzymatic activity was localized predominantly in the cytosolic fractions, it was dependent on NADPH and Ca2+, and inhibited by N(G)-nitro-L-arginine or N(G)-methyl-L-arginine. 3 Western blot analysis revealed that all regions of the ferret brain contained a 160 kD protein crossreacting with an antibody to nitric oxide synthase purified from the rat cerebellum, and the levels of relative intensity of staining by the antibody correlated with the distribution of nitric oxide synthase activity. 4 These results indicate that the ferret brain contains a nitric oxide snythase similar to the rat brain, but the distribution of enzymatic activity in the ferret brain differs markedly from the rat brain.
引用
收藏
页码:849 / 852
页数:4
相关论文
共 20 条
  • [1] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [2] NITRIC-OXIDE, A NOVEL NEURONAL MESSENGER
    BREDT, DS
    SNYDER, SH
    [J]. NEURON, 1992, 8 (01) : 3 - 11
  • [3] NITRIC-OXIDE SYNTHASE PROTEIN AND MESSENGER-RNA ARE DISCRETELY LOCALIZED IN NEURONAL POPULATIONS OF THE MAMMALIAN CNS TOGETHER WITH NADPH DIAPHORASE
    BREDT, DS
    GLATT, CE
    HWANG, PM
    FOTUHI, M
    DAWSON, TM
    SNYDER, SH
    [J]. NEURON, 1991, 7 (04) : 615 - 624
  • [4] ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME
    BREDT, DS
    SNYDER, SH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) : 682 - 685
  • [5] CALMODULIN-DEPENDENT ENDOTHELIUM-DERIVED RELAXING FACTOR NITRIC-OXIDE SYNTHASE ACTIVITY IS PRESENT IN THE PARTICULATE AND CYTOSOLIC FRACTIONS OF BOVINE AORTIC ENDOTHELIAL-CELLS
    FORSTERMANN, U
    POLLOCK, JS
    SCHMIDT, HHHW
    HELLER, M
    MURAD, F
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) : 1788 - 1792
  • [6] REGIONAL DISTRIBUTION OF EDRF NO-SYNTHESIZING ENZYME(S) IN RAT-BRAIN
    FORSTERMANN, U
    GORSKY, LD
    POLLOCK, JS
    SCHMIDT, HHHW
    HELLER, M
    MURAD, F
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 168 (02) : 727 - 732
  • [7] ISOFORMS OF NITRIC-OXIDE SYNTHASE - CHARACTERIZATION AND PURIFICATION FROM DIFFERENT CELL-TYPES
    FORSTERMANN, U
    SCHMIDT, HHHW
    POLLOCK, JS
    SHENG, H
    MITCHELL, JA
    WARNER, TD
    NAKANE, M
    MURAD, F
    [J]. BIOCHEMICAL PHARMACOLOGY, 1991, 42 (10) : 1849 - 1857
  • [8] ENDOTHELIUM-DERIVED RELAXING FACTOR RELEASE ON ACTIVATION OF NMDA RECEPTORS SUGGESTS ROLE AS INTERCELLULAR MESSENGER IN THE BRAIN
    GARTHWAITE, J
    CHARLES, SL
    CHESSWILLIAMS, R
    [J]. NATURE, 1988, 336 (6197) : 385 - 388
  • [9] GLUTAMATE, NITRIC-OXIDE AND CELL CELL SIGNALING IN THE NERVOUS-SYSTEM
    GARTHWAITE, J
    [J]. TRENDS IN NEUROSCIENCES, 1991, 14 (02) : 60 - 67
  • [10] ISHII K, 1989, J APPL CARDIOL, V4, P505