PARTIAL-PURIFICATION OF FARNESYL PYROPHOSPHATE - DRIMENOL CYCLASE AND GERANYLGERANYL PYROPHOSPHATE - SCLAREOL CYCLASE, USING CELL-CULTURE AS A SOURCE OF MATERIAL

被引：16

作者：

BANTHORPE, DV ^{[1
]}

BROWN, JT ^{[1
]}

MORRIS, GS ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL, DEPT CHEM, LONDON WC1H 0AJ, ENGLAND

来源：

PHYTOCHEMISTRY | 1992年 / 31卷 / 10期

关键词：

FARNESYL PYROPHOSPHATE; DRIMENOL CYCLASE; GERANYLGERANYL PYROPHOSPHATE; SCLAREOL CYCLASE; TISSUE CULTURE; ENZYME PURIFICATION;

D O I：

10.1016/0031-9422(92)83692-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell cultures of Polygonum hydropiper and Nicotiana glutinosa provide convenient material for the purification of farnesyl pyrophosphate: drimenol cyclase and geranylgeranyl pyrophosphate: sclareol cyclase, respectively. Similar three-step procedures from homogenates of the cultured cells give purification of 2850-and 625-fold, respectively. An improved method for the preparation of phosphorylated terpenoid intermediates for use as substrates is also described.

引用

页码：3391 / 3395

页数：5

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