AUTOMATED COLUMN LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMOXICILLIN AND CEFADROXIL IN BOVINE SERUM AND MUSCLE-TISSUE USING ONLINE DIALYSIS FOR SAMPLE PREPARATION

被引:30
作者
SNIPPE, N
VANDEMERBEL, NC
RUITER, FPM
STEIJGER, OM
LINGEMAN, H
BRINKMAN, UAT
机构
[1] FREE UNIV AMSTERDAM,DEPT ANALYT CHEM,1081 HV AMSTERDAM,NETHERLANDS
[2] VET RES INST,8090 AB WEZEP,NETHERLANDS
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 662卷 / 01期
关键词
D O I
10.1016/0378-4347(94)00377-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A fully automated method is described for the determination of amoxicillin and cefadroxil in bovine serum and muscle tissue. The method is based on the on-line combination of dialysis and solid-phase extraction for sample preparation, and column liquid chromatography with ultraviolet detection. In order to enhance the UV detectability of the analytes, post-column addition of 0.1 M sodium hydroxide is performed. The method shows good linearity and repeatability for both analytes in serum as well as in muscle tissue; the limits of detection in these samples are 0.05 mu g/ml and 0.2 mu g/g, respectively. The method has a sample throughput of 30 samples per 24 h.
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收藏
页码:61 / 70
页数:10
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