PRACTICAL RADIOCHROMATOGRAPHIC ASSAY FOR CHOLESTEROL EPOXIDE HYDRASE

被引：8

作者：

BLACK, HS

LENGER, WA

机构：

[1] BAYLOR UNIV,COLL MED,DEPT DERMATOL,HOUSTON,TX 77030

[2] BAYLOR UNIV,COLL MED,DEPT BIOCHEM,HOUSTON,TX 77030

[3] VET ADM HOSP,PHOTOBIOL LAB,HOUSTON,TX 77211

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90377-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the assay of cholesterol epoxide hydrase activity is described. The assay involves the thin-layer chromatographic separation and quantitation of radiolabeled cholestan-3β,5α,6α-epoxide and its major hydration product, cholestan-3β,5α,6β-triol. Radiochromatographic scanning is employed to quantitate the reaction. The procedure is sensitive, rapid, and nondestructive. © 1979.

引用

页码：383 / 385

页数：3

共 18 条

[1]

Bischoff F, 1969, Adv Lipid Res, V7, P165

[2]

BLACK HS, 1973, CANCER RES, V33, P2094

[3] FORMATION OF A CARCINOGEN IN HUMAN SKIN IRRADIATED WITH ULTRAVIOLET LIGHT [J].