学术探索
学术期刊
新闻热点
数据分析
智能评审

BASE COMPOSITION HETEROGENEITY OF EUGLENA-GRACILIS CHLOROPLAST DNA

被引：6
作者：
GRAY, PW
HALLICK, RB
机构：
[1] Department of Chemistry, University of Colorado, Boulder
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 01期
基金：
美国国家卫生研究院;
关键词：
(Euglena); Base composition; Chloroplast DNA; Restriction nuclease; rRNA gene;
D O I：
10.1016/0005-2787(79)90489-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Euglina gracilis chloroplast DNA has an average buoyant density of 1.685 gm/cm3, corresponding to 25 mol% G · C base pairs. To test for base compositional heterogeneity within this 130 kilobase pairs (kbp) genome, previously mapped restriction endonuclease fragments were isolated, and characterized by equilibrium buoyant density centrifugation. The chloroplast DNA can be characterized as containing two major buoyant density components. A segment of 17 kbp, representing 13% of the genome and containing the rRNA genes is 43-44 mol% G · C. The remaining 113 kbp, accounting for 87% of the genome, has an average 20-21 mol% G · C content. © 1979.
引用
收藏
页码：53 / 58
页数：6
相关论文
共 21 条
123