OXIDATION OF 2-KETO-4-HYDROXYGLUTARATE BY PIG HEART AND ESCHERICHIA-COLI ALPHA-KETOGLUTARATE DEHYDROGENASE COMPLEX

被引：2

作者：

GUPTA, SC

DEKKER, EE

机构：

[1] Department of Biological Chemistry The University of Michigan, Ann Arbor

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 192卷 / 01期

关键词：

D O I：

10.1016/0003-9861(79)90099-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzyme preparations from pig heart and Escherichia coli have been found to catalyze a NAD+- and CoASH-dependent oxidation of 2-keto-4-hydroxyglutarate. Several independent lines of evidence indicate that 2-keto-4-hydroxyglutarate is a substrate for the well-known α-ketoglutarate dehydrogenase complex of the citric acid cycle. The evidence includes (a) a constant ratio of specific activity values for the two substrates throughout purification, (b) identical elution profiles from a Ca3(PO4)2 gel-cellulose column, (c) the same sucrose density sedimentation patterns, (d) similar responses in controlled heat inactivation studies, and (e) identical pH-activity curves. © 1979.

引用

页码：324 / 326

页数：3

共 12 条

[1]

ADAMS E, 1960, J BIOL CHEM, V235, P3492

[2]

ADAMS E, 1960, J BIOL CHEM, V235, P3504

[3]

DEKKER EE, 1962, J BIOL CHEM, V237, P2218

[4]

KOBES RD, 1969, J BIOL CHEM, V244, P1919

[5]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[6]

MAITRA U, 1963, J BIOL CHEM, V238, P3660

[7]

MARTIN RG, 1961, J BIOL CHEM, V236, P1372

[8] THE COMPOSITION OF THE KETOGLUTARATE DEHYDROGENASE COMPLEX [J].

MASSEY, V .

BIOCHIMICA ET BIOPHYSICA ACTA, 1960, 38 (03) :447-460

[9]

NISHIHARA H, 1972, J BIOL CHEM, V247, P5079

[10] ENZYMATIC CONVERSION OF DELTA-OH-ALPHA-KETOGLUTARATE (HKG) TO MALATE - (A POSTULATED STEP IN CYCLIC OXIDATION OF GLYOXYLATE) [J].

PAYES, B ;

LATIES, GG .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1963, 13 (03) :179-&

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