DETERMINATION OF PROTEINS IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH SPECTROPHOTOMETRIC DETECTION USING A PYROGALLOL RED MOLYBDATE COMPLEX

被引：3

作者：

MASHIGE, F

OHKUBO, A

机构：

[1] Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, 113

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80381-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method with spectrophotometric detection was developed for the determination of proteins in urine. The proteins were separated on an anion-exchange column and eluted with a Tris-HCl buffer with a gradient of sodium chloride concentration and pH. The separated proteins were mixed with a pyrogallol red-molybdate complex reagent and determined spectrophotometrically. Urinary proteins were well separated without desalting the urine. The reproducibility was satisfactory.

引用

页码：173 / 181

页数：9

共 14 条

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[2]

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