EVIDENCE FOR CROSS-LINKING DNA BY BIS-INTERCALATORS WITH RIGID AND EXTENDED LINKERS IS PROVIDED BY KNOTTING AND CATENATION

被引:18
作者
ANNAN, NK
COOK, PR
MULLINS, ST
LOWE, G
机构
[1] UNIV OXFORD, DYSON PERRINS LAB, S PARKS RD, OXFORD OX1 3QY, ENGLAND
[2] UNIV OXFORD, SIR WILLIAM DUNN SCH PATHOL, OXFORD OX1 3RE, ENGLAND
[3] BRUNEL UNIV, DEPT CHEM, UXBRIDGE UB8 3PH, MIDDX, ENGLAND
关键词
D O I
10.1093/nar/20.5.983
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new series of DNA bis-intercalators is reported in which acridine moieties are connected by rigid and extended pyridine-based linkers of varied length. Cross-linking of DNA by bis-intercalation is inferred from the unwinding and folding of linear DNA induced by the compounds; after ligation and removal of the bis-intercalator, superhelical circles, catenanes and knots that bear a residual imprint of the bis-intercalator are observed. These novel bis-intercalators are of interest because they can be used to probe the spatial organization of DNA, especially near sites of replication, recombination or topoisomerase action where two duplexes must be in close proximity. Preliminary results on the effects of the various compounds on the cloning efficiency of bacteria and replication by permeabilized human cells are also presented.
引用
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页码:983 / 990
页数:8
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