IDENTIFICATION AND CHARACTERIZATION OF 2 RECEPTORS FOR SCHISTOFLRFAMIDE ON LOCUST OVIDUCT

被引：26

作者：

WANG, ZX ^{[1
]}

ORCHARD, I ^{[1
]}

LANGE, AB ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT ZOOL,TORONTO M5S 1A1,ON,CANADA

来源：

PEPTIDES | 1994年 / 15卷 / 05期

基金：

加拿大自然科学与工程研究理事会;

关键词：

FMRFAMIDE-RELATED PEPTIDES; BINDING SITES; BINDING ASSAY; LOCUSTA MIGRATORIA; INSECT VISCERAL MUSCLE; OVIDUCT MEMBRANE;

D O I：

10.1016/0196-9781(94)90045-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An in vitro binding assay, using [I-125][Y-1]SchistoFLRFamide (YDVDHVFLRFamide), an iodinated analogue of SchistoFLRFamide, was developed to demonstrate and characterize putative receptors for SchistoFLRFamide associated with the locust oviduct. Two receptors were revealed by the binding assay. The high-affinity receptor had a K-d of 9.52 +/- 1.15 X 10(-10) M and a B-max of 14.5 +/- 1.2 fmol/mg membrane protein; the low-affinity receptor had a K-d of 1.86 +/- 0.16 x 10(-7) M and a B-max of 540 +/- 43 fmol/mg membrane protein. Binding to both receptors was saturable, specific, and reversible, and competitively inhibited by [Y-1]SchistoFLRFamide, SchistoFLRFamide, and ADVGHVFLRFamide. Binding was Ca2+ or Mg2+ dependent and was completely inhibited by 2 mM EDTA. The receptors showed a regional distribution, with the majority of the high-affinity receptors associated with the upper lateral oviducts, which receive little or no innervation, and most of the low-affinity receptors associated with the lower lateral and common oviducts, which receive extensive innervation.

引用

页码：875 / 882

页数：8

共 29 条

[1] CHARACTERIZATION OF RAT SPINAL-CORD RECEPTORS TO FLFQPQRFAMIDE, A MAMMALIAN MORPHINE MODULATING PEPTIDE - A BINDING STUDY [J].