PURIFICATION AND CHARACTERIZATION OF CYTOCHROME-P450 FROM AN ISOBUTENE-FORMING MICROORGANISM, RHODOTORULA-MINUTA

被引：6

作者：

FUKUDA, H ^{[1
]}

FUJII, T ^{[1
]}

DAIMON, H ^{[1
]}

IWATA, M ^{[1
]}

OGAWA, T ^{[1
]}

TANASE, S ^{[1
]}

MORINO, Y ^{[1
]}

机构：

[1] KUMAMOTO UNIV, SCH MED, DEPT BIOCHEM, KUMAMOTO 860, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 09期

关键词：

D O I：

10.1271/bbb.57.1599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cytochrome P450 was purified from microsomes of Rhodotorula minuta. The optical spectrum of the purified cytochrome was characteristic of a low-spin ferric heme protein. Isovalerate caused a type I spectral change in it. The amino-terminal sequence of the cytochrome was different from those of other known microsomal cytochrome P450s. These results indicate that the cytochrome, which is tentatively named P450rm, is a novel species of cytochrome P450.

引用

页码：1599 / 1601

页数：3

共 16 条

[1]

BLACK SD, 1987, ADV ENZYMOL RAMB, V60, P35

[2] SEQUENCE-ANALYSIS OF RIPENING-RELATED CYTOCHROME-P-450 CDNAS FROM AVOCADO FRUIT [J].

BOZAK, KR ;

YU, H ;

SIREVAG, R ;

CHRISTOFFERSEN, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) :3904-3908

[3]

FUJII T, 1987, APPL MICROBIOL BIOT, V25, P430

[4] THE RELATIONSHIP BETWEEN ISOBUTENE-FORMING ACTIVITY AND A CYTOCHROME-P-450 IN RHODOTORULA-MINUTA [J].

FUJII, T ;

OGAWA, T ;

FUKUDA, H .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1989, 68 (03) :174-177

[5] THE ROLE OF L-PHENYLALANINE IN THE PRODUCTION OF ISOBUTENE BY RHODOTORULA-MINUTA [J].

FUJII, T ;

OHGA, Y ;

OGAWA, T ;

FUKUDA, H .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1989, 67 (02) :115-118

[6]

FUJII T, 1989, FEMS MICROBIOL LETT, V58, P199

[7]

FUJII T, 1988, APPL ENVIRON MICROB, V5, P583

[8]

IMAI Y, 1987, SAIBOU KOUGAKU, V6, P98

[9]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[10]

OMURA T, 1964, J BIOL CHEM, V239, P2379

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