Degradation of 2,4,5-Trichlorophenoxyacetic acid by a Nocardioides simplex culture

被引:34
作者
Golovleva, Ludmila A. [1 ]
Pertsova, Raisa N. [1 ]
Evtushenko, Ludmila I. [1 ]
Baskunov, Boris P. [1 ]
机构
[1] USSR Acad Sci, Inst Biochem & Physiol Microorganisms, Pushchino 142292, Moscow Region, Russia
关键词
adaptation; dechlorination; 2,4-dichlorophenoxyacetic acid; isolation; Nocardioides; 2,4,5-trichlorophenol; 2,4,5-trichlorophenoxyacetic acid; utilization;
D O I
10.1007/BF00119763
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A Nocardioides simplex strain 3E was isolated which totally dechlorinated 2,4,5-trichlorophenoxyacetic acid and was capable of its utilization as the sole source of carbon. The mechanism of 2,4,5-trichlorophenoxyacetic acid degradation by this strain was investigated. Chloroaromatic metabolites that occur in the lag, exponential and stationary growth phases of the strain Nocardioides simplex 3E were isolated and identified bases on a combination of TLC, GC-MS and HPLC data. Decomposition of 2,4,5-trichlorophenoxyacetic acid at the initial stage was shown to proceed by two pathways: via the splitting of the two-carbon fragment to yield 2,4,5-trichlorophenol and the reductive dechlorination to produce 2,4-dichlorophenoxyacetic acid. Hydrolytic dechlorination of 2,4,5-trichlorophenoxyacetic acid was found to yield dichlorohydroxyphenoxyacetic acid, thus pointing to the possible existence of a third branch at the initial stage of degradation of the xenobiotic. 2,4,5-Trichlorophenol and 2,4-dichlorophenoxyacetic acid produced during the metabolism of 2,4,5-trichlorophenoxyacetic acid and in experiments with resting cells are utilized by the strain Nocardioides simplex 3E as growth substrates.
引用
收藏
页码:263 / 271
页数:9
相关论文
共 22 条
[1]   COMPLETE DECHLORINATION OF TETRACHLOROHYDROQUINONE BY CELL-EXTRACTS OF PENTACHLOROPHENOL-INDUCED RHODOCOCCUS-CHLOROPHENOLICUS [J].
APAJALAHTI, JHA ;
SALKINOJASALONEN, MS .
JOURNAL OF BACTERIOLOGY, 1987, 169 (11) :5125-5130
[2]   GENETIC HOMOLOGIES AMONG NOCARDIAE AND OTHER ACTINOMYCETES [J].
BRADLEY, SG ;
BROWNELL, GH ;
CLARK, J .
CANADIAN JOURNAL OF MICROBIOLOGY, 1973, 19 (08) :1007-1014
[3]  
Chakrabarty AM, 1987, MICROBIAL GENETIC EN
[4]  
COLLINS MD, 1989, FEMS MICROBIOL LETT, V57, P289
[5]   TRANSFER OF PIMELOBACTER-TUMESCENS TO TERRABACTER GEN-NOV AS TERRABACTER-TUMESCENS COMB NOV AND OF PIMELOBACTER-JENSENII TO NOCARDIOIDES AS NOCARDIOIDES-JENSENII COMB NOV [J].
COLLINS, MD ;
DORSCH, M ;
STACKEBRANDT, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (01) :1-6
[6]  
Collins MD., 1985, METHOD MICROBIOL, V18, P329, DOI 10.1016/S0580-9517(08)70480-X
[7]  
FARROW JAE, 1983, J GEN MICROBIOL, V129, P1423
[8]   NUMERICAL TAXONOMY OF SOME NOCARDIOFORM BACTERIA [J].
GOODFELLOW, M .
JOURNAL OF GENERAL MICROBIOLOGY, 1971, 69 (NOV) :33-+
[9]   NOCARDIA-COELIACA, NOCARDIA-AUTOTROPHICA, AND NOCARDIN STRAIN [J].
GORDON, RE ;
BARNETT, DA ;
HANDERHAN, JE ;
PANG, CHN .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1974, 24 (01) :54-63
[10]   RAPID METHOD FOR DISTINCTION OF GRAM-NEGATIVE FROM GRAM-POSITIVE BACTERIA [J].
GREGERSEN, T .
EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 5 (02) :123-127