A NOVEL SITE-SPECIFIC RECOMBINASE ENCODED BY THE STREPTOCOCCUS-PYOGENES PLASMID PSM19035

Genetic evidence suggests that the β protein encoded by the Streptococcus pyogenes plasmid pSM19035 is involved both in the resolution of plasmid multimers into monomers and in DNA inversion. In this report we show that the highly purified β protein is unable to mediate DNA recombination unlessa host factor(s) is provided. In the presence of the host factor(s), the β protein is able to catalyze in vitro intramolecular recombination between twospecific sites on supercoiled templates: DNA resolution was obtained when the two recombination sites weredirectly oriented, whereas DNA inversion was the product if the recombination sites were in inverse orientation. In the absence of the host factor(s) the β protein forms a specific complex with its target site. The 15 protein binding site has been localized by DNase I footprinting to an 85 bp region that can be divided into two discrete sites (I and II). These sites are about 34 bp in length, they are separated byabout 16 bp, and contain two 12 to 13 bp imperfectly conserved sequences (half-sites) with dyad axis symmetry. The protein binds co-operatively to sites I and II; between 3·6 and 4·2 β protein protomers are required to saturate the DNA substrate. These data, together with gel retardation assays, suggest that the protein binds to DNA as two dimers, one to each discrete site, and that the dimers probably interact with each other. The β proteinbinding site, though it resembles that of other DNA resolvases of the Tn3/γδ (Tn1000) family, differs in that only two adjacent sites are found (sites I and II), while those DNA resolvases normallybind to three adjacent sites. The results presented here suggest that a host factor(s) could work as an accessory effector to compensate for the absence of thethird binding site. © 1994 Academic Press, Inc.