FACTORS AFFECTING THE ACYLTRANSFER ACTIVITY OF THE LIPASE FROM CANDIDA-PARAPSILOSIS IN AQUEOUS-MEDIA

被引：20

作者：

BRIAND, D ^{[1
]}

DUBREUCQ, E ^{[1
]}

GALZY, P ^{[1
]}

机构：

[1] INRA,ENSAM,MICROBIOL IND & GENET MICROORGANISMES LAB,F-34060 MONTPELLIER 1,FRANCE

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1995年 / 72卷 / 11期

关键词：

ACYLTRANSFERASE; ALCOHOLYSIS; CANDIDA PARAPSILOSIS; HYDROLYSIS; LIPASE;

D O I：

10.1007/BF02546213

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study describes the influence of various factors on the hydrolysis and alcoholysis activities of the lipase from Candida parapsilosis (Ashford), Langeron and Talice in aqueous media. Optimal activities were obtained at 45 degrees C and pH 6-6.5. The influence of the nature of the substrate on the temperature activity profiles was observed. Total or partial recovery of the activities was obtained when methanol was added to the enzyme extract after thermal denaturation. A tyrosin residue appeared to be necessary for lipase function. Magnesium was a required metal cofactor. These activities were optimal in the presence of high amounts of water (water activity > 0.9).

引用

页码：1367 / 1373

页数：7

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