CLONING AND NUCLEOTIDE SEQUENCING OF THE TYROSINE PHENOL LYASE GENE FROM ESCHERICHIA-INTERMEDIA

被引:12
作者
KURUSU, Y [1 ]
FUKUSHIMA, M [1 ]
KOHAMA, K [1 ]
KOBAYASHI, M [1 ]
TERASAWA, M [1 ]
KUMAGAI, H [1 ]
YUKAWA, H [1 ]
机构
[1] KYOTO UNIV,DEPT FOOD SCI & TECHNOL,KYOTO 606,JAPAN
关键词
D O I
10.1007/BF01026756
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The tyrosine phenol lyase (TPL) gene was cloned from the genomic DNA of a Escherichia intermedia strain and the nucleotide sequence of the TPL structural gene was determined. The 1801 bp HincII-NruI DNA fragment containing the TPL gene had an open reading frame of 1368 bp and the deduced amino acid sequence was 456 residues long with a molecular weight of 51,441 daltons.
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页码:769 / 772
页数:4
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