PHOSPHOLIPASE-D TREATMENT ENHANCES GONADOTROPIN RECEPTOR-COUPLED ADENYLATE-CYCLASE ACTIVITY IN ISOLATED BOVINE LUTEAL CELLS

被引：9

作者：

BUDNIK, LT ^{[1
]}

MUKHOPADHYAY, AK ^{[1
]}

机构：

[1] UNIV HAMBURG,INST HORMONE & FERTIL RES,GRANDWEG 64,W-2000 HAMBURG 54,GERMANY

来源：

FEBS LETTERS | 1993年 / 326卷 / 1-3期

关键词：

PHOSPHATIDIC ACID; CYCLIC AMP; PHORBOL ESTER; PROTEIN KINASE-C; SIGNAL TRANSDUCTION;

D O I：

10.1016/0014-5793(93)81795-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LH-stimulated adenylate cyclase activity in membrane preparations of bovine luteal cells could be enhanced by treating the cells with either phospholipase D or its hydrolysis product, phosphatidic acid. Similar augmentary effects were also produced following treatment of the cells with EGF. Moreover, EGF could stimulate the formation of [H-3]phosphatidic acid in [H-3]myristic acid preloaded cells, suggesting that EGF is able to activate cellular phospholipase D. Also, PMA was able to increase the phosphatidic acid formation with a parallel increase in the adenylate cyclase, activity. We propose, therefore, that phosphatidic acid may act as an intracellular second messenger finking EGF-mediated activation of phospholipase D with the sensitization of LH receptor-coupled adenylate cyclase signalling system.

引用

页码：222 / 226

页数：5

共 28 条

[1]

AGWU DE, 1989, J BIOL CHEM, V264, P1405

[2]

BALL RL, 1990, J BIOL CHEM, V265, P12836

[3]

BILIGH EG, 1959, CAN J BIOCH PHYSL, V37, P911

[4] THE REGULATION AND CELLULAR FUNCTIONS OF PHOSPHATIDYLCHOLINE HYDROLYSIS [J].

BILLAH, MM ;

ANTHES, JC .

BIOCHEMICAL JOURNAL, 1990, 269 (02) :281-291

[5] PHOSPHATIDATE-DEPENDENT PROTEIN-PHOSPHORYLATION [J].

BOCCKINO, SB ;

WILSON, PB ;

EXTON, JH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (14) :6210-6213

[6] EPIDERMAL GROWTH-FACTOR MIMICS INSULIN EFFECTS IN RAT HEPATOCYTES [J].

BOSCH, F ;

BOUSCAREL, B ;

SLATON, J ;

BLACKMORE, PF ;

EXTON, JH .

BIOCHEMICAL JOURNAL, 1986, 239 (03) :523-530

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] PHORBOL ESTER STIMULATES PROGESTERONE PRODUCTION BY ISOLATED BOVINE LUTEAL CELLS [J].

BRUNSWIG, B ;

MUKHOPADHYAY, AK ;

BUDNIK, LT ;

BOHNET, HG ;

LEIDENBERGER, FA .

ENDOCRINOLOGY, 1986, 118 (02) :743-749

[9] DESENSITIZATION OF LH-STIMULATED CYCLIC-AMP ACCUMULATION IN ISOLATED BOVINE LUTEAL CELLS - EFFECT OF PHORBOL ESTER [J].

BUDNIK, LT ;

MUKHOPADHYAY, AK .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1987, 54 (01) :51-61

[10]

BUDNIK LT, 1991, J BIOL CHEM, V266, P13908

← 1 2 3 →