A RAPID AND EFFICIENT PCR-BASED METHOD FOR SYNTHESIZING HIGH-MOLECULAR-WEIGHT MULTIMERS OF OLIGONUCLEOTIDES

被引：19

作者：

HEMAT, F

MCENTEE, K

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,STRUCT BIOL & MOLEC MED LAB,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,SCH MED,DEPT BIOL CHEM,LOS ANGELES,CA 90024

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2690

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30 - 100 repeating units. (C) 1994 Academic Press, Inc.

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页码：475 / 481

页数：7

相关论文

共 8 条

[1] KEY FEATURES OF HEAT-SHOCK REGULATORY ELEMENTS [J].

AMIN, J ;

ANANTHAN, J ;

VOELLMY, R .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (09) :3761-3769

[2] PURIFICATION AND CHARACTERIZATION OF OBF1 - A SACCHAROMYCES-CEREVISIAE PROTEIN THAT BINDS TO AUTONOMOUSLY REPLICATING SEQUENCES [J].

FRANCESCONI, SC ;

EISENBERG, S .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (07) :2906-2913

[3] PURIFICATION AND FUNCTIONAL-CHARACTERIZATION OF A CELLULAR TRANSCRIPTION FACTOR THAT BINDS TO AN ENHANCER ELEMENT WITHIN THE ADENOVIRUS EARLY EIIA PROMOTER [J].

JALINOT, P ;

WINTZERITH, M ;

GAIRE, M ;

HAUSS, C ;

EGLY, JM ;

KEDINGER, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (08) :2484-2488

[4] AFFINITY PURIFICATION OF SEQUENCE-SPECIFIC DNA-BINDING PROTEINS [J].

KADONAGA, JT ;

TJIAN, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (16) :5889-5893

[5] PURIFICATION OF A SET OF CELLULAR POLYPEPTIDES THAT BIND TO THE PURINE-RICH CIS-REGULATORY ELEMENT OF HERPES-SIMPLEX VIRUS IMMEDIATE EARLY GENES [J].

LAMARCO, KL ;

MCKNIGHT, SL .

GENES & DEVELOPMENT, 1989, 3 (09) :1372-1383

[6] PURIFICATION OF THE HETEROMERIC PROTEIN-BINDING TO THE URS1 TRANSCRIPTIONAL REPRESSION SITE IN SACCHAROMYCES-CEREVISIAE [J].

LUCHE, RM ;

SMART, WC ;

COOPER, TG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) :7412-7416

[7]

ROSENFELD PJ, 1986, J BIOL CHEM, V261, P1398

[8] PURIFICATION AND CHARACTERIZATION OF A HEAT-SHOCK ELEMENT BINDING-PROTEIN FROM YEAST [J].

SORGER, PK ;

PELHAM, HRB .

EMBO JOURNAL, 1987, 6 (10) :3035-3041