CLONING AND FUNCTIONAL EXPRESSION OF A CDNA-ENCODING COFFEE BEAN ALPHA-GALACTOSIDASE

被引：67

作者：

ZHU, A ^{[1
]}

GOLDSTEIN, J ^{[1
]}

机构：

[1] NEW YORK BLOOD CTR,LINDSLEY F KIMBALL RES INST,310 E 67TH ST,NEW YORK,NY 10021

来源：

GENE | 1994年 / 140卷 / 02期

关键词：

SEQUENCING; ERYTHROCYTE SURFACE; GLYCOSIDASE; RECOMBINANT DNA; B-]O BLOOD GROUP CONVERSION; INSECT CELLS;

D O I：

10.1016/0378-1119(94)90548-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purified coffee bean alpha-galactosidase (alphaGal) has been used for removing terminal alpha-galactose residues from the glycoconjugates at the red cell surface, in studies of blood group conversion. Here, we report the isolation and sequence of the full-length clone for coffee bean alphaGal by using the polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) techniques. The cDNA clone (1.4 kb) contains a single open reading frame which encodes a protein of 378 amino acids (aa). Its authenticity is confirmed by perfect alignment of aa sequences obtained from purified coffee bean alphaGal, and by immune reaction with the antibody raised against the enzyme. Furthermore, the protein produced in insect cells shows enzymatic activity towards a synthetic alphaGal substrate, p-nitro-phenyl-alpha-galactopyranoside.

引用

页码：227 / 231

页数：5

共 16 条

[1] CORRECTION [J].

ADUSEOPOKU, J .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :2271-2271

[2] HUMAN ALPHA-GALACTOSIDASE-A - NUCLEOTIDE-SEQUENCE OF A CDNA CLONE ENCODING THE MATURE ENZYME [J].

BISHOP, DF ;

CALHOUN, DH ;

BERNSTEIN, HS ;

HANTZOPOULOS, P ;

QUINN, M ;

DESNICK, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (13) :4859-4863

[3] CLONING AND EXPRESSION OF A MEMBER OF THE ASPERGILLUS-NIGER GENE FAMILY ENCODING ALPHA-GALACTOSIDASE [J].

DENHERDER, IF ;

ROSELL, AMM ;

VANZUILEN, CM ;

PUNT, PJ ;

VANDENHONDEL, AMJJ .

MOLECULAR & GENERAL GENETICS, 1992, 233 (03) :404-410

[4] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[5] GROUP-B ERYTHROCYTES ENZYMATICALLY CONVERTED TO GROUP-O SURVIVE NORMALLY IN A, B, AND O INDIVIDUALS [J].

GOLDSTEIN, J ;

SIVIGLIA, G ;

HURST, R ;

LENNY, L ;

REICH, L .

SCIENCE, 1982, 215 (4529) :168-170

[6]

Goldstein J, 1989, Transfus Med Rev, V3, P206, DOI 10.1016/S0887-7963(89)70080-8

[7]

GUISEPPIN ML, 1993, APPL ENVIRON MICROB, V59, P52

[8]

HAMA H, 1992, J BIOL CHEM, V267, P18371

[9]

King L.A., 1992, BACULOVIRUS EXPRESSI

[10]

LENNY LL, 1991, BLOOD, V77, P1383

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