HEPATITIS-E VIRUS (87A STRAIN) PROPAGATED IN A549 CELLS .1.

被引：41

作者：

HUANG, RT

NAKAZONO, N

ISHII, K

LI, DR

KAWAMATA, O

KAWAGUCHI, R

TSUKADA, Y

机构：

[1] KANSAI MED UNIV,DEPT PUBL HLTH,MORIGUCHI,OSAKA 570,JAPAN

[2] SPECIAL RES LAB,GENET RES LAB,HACHIOJI,TOKYO,JAPAN

[3] BEIJING INST MICROBIOL & EPIDEMIOL,BEIJING,PEOPLES R CHINA

来源：

JOURNAL OF MEDICAL VIROLOGY | 1995年 / 47卷 / 04期

关键词：

HEPATITIS E VIRUS; CELL CULTURE; A549 CELL LINE; POLYMERASE CHAIN REACTION;

D O I：

10.1002/jmv.1890470402

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A strain of hepatitis E virus (HEV), the 87A strain isolated in 2BS cells from the feces of a patient with hepatitis E, has been reported previously. In this study, the 87A strain was propagated in A549 cells, and the marked cytopathic effect (CPE) appeared in the infected monolayer cells. The size of this virus is about 30 nm in diameter. Furthermore, HEV-RNA from the supernatants of the virus of different passages was detected by polymerase chain reaction (PCR) amplification using ET1.1 HEV primers. A band of HEV for 239 bp from PCR products was revealed by electro-phoresis. PCR products of the fourth passage were sequenced. These results show that the 87A virus replicates in the A549 cell line. (C) 1995 Wiley-Liss, Inc.

引用

页码：299 / 302

页数：4

共 12 条

[1] EVIDENCE FOR A VIRUS IN NON-A, NON-B HEPATITIS TRANSMITTED VIA THE FECAL-ORAL ROUTE [J].