SIMULTANEOUS PRODUCTION AND PARTITION OF CHITINASE DURING GROWTH OF SERRATIA-MARCESCENS IN AN AQUEOUS 2-PHASE SYSTEM

被引：16

作者：

CHEN, JP ^{[1
]}

LEE, MS ^{[1
]}

机构：

[1] NATL CHENG KUNG UNIV,DEPT CHEM ENGN,TAINAN 701,TAIWAN

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 11期

关键词：

D O I：

10.1007/BF00152884

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Partition and production of the extracellular chitinase from Serratia marcescens were studied in PEG/dextran aqueous two-phase systems. The enzyme partitions into the bottom phase and the cells segregate into the top phase. The best system is 2% (w/v) PEG 20000 and 5% (w/v) dextran T500. The cell growth and enzyme production kinetics are similar in the aqueous two-phase system and in the polymer-free reference system. However, the maximum enzyme concentration in the former system is 1.5 times that in the latter one.

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页码：783 / 788

页数：6

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