DNA-FINGERPRINTING OF PSEUDOMONAS-AERUGINOSA SEROTYPE O-11 BY ENTEROBACTERIAL REPETITIVE INTERGENIC CONSENSUS POLYMERASE CHAIN-REACTION AND PULSED-FIELD GEL-ELECTROPHORESIS

被引：15

作者：

LAU, YJ ^{[1
]}

LIU, PYF ^{[1
]}

HU, BS ^{[1
]}

SHYR, JM ^{[1
]}

SHI, ZY ^{[1
]}

TSAI, WS ^{[1
]}

LIN, YH ^{[1
]}

TSENG, CY ^{[1
]}

机构：

[1] TAICHUNG VET GEN HOSP,INFECT DIS SECT,TAICHUNG,TAIWAN

来源：

JOURNAL OF HOSPITAL INFECTION | 1995年 / 31卷 / 01期

关键词：

PSEUDOMONAS AERUGINOSA O-11; PULSED-FIELD GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0195-6701(95)90084-5

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We report the use of pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus (ERIC)-based polymerase chain reaction (PCR) to characterize clinical isolates of Pseudomonas aeruginosa serotype 011 collected from an incident of hospital-acquired infection. Both typing techniques differentiated 20 different strain types among seven epidemiologically related isolates and 22 epidemiologically unrelated isolates. There was complete concordance between these two techniques. Our results indicate that the ERIC-based PCR technique represents a rapid and simple means for typing P. aeruginosa serotype 011 with a level of discrimination equivalent to that of PFGE.

引用

页码：61 / 66

页数：6

共 6 条

[1]

BROKOPP CD, 1977, J CLIN MICROBIOL, V5, P640

[2] USE OF PCR TO STUDY EPIDEMIOLOGY OF SERRATIA-MARCESCENS ISOLATES IN NOSOCOMIAL INFECTION [J].