METHOD FOR PREPARING AND HANDLING CROSS-SECTIONS FROM EARLY CHICK-EMBRYOS FOR SCANNING ELECTRON-MICROSCOPY

被引：7

作者：

NAGELE, RG ^{[1
]}

GOLDSTEIN, MM ^{[1
]}

LEE, H ^{[1
]}

机构：

[1] RUTGERS STATE UNIV, DEPT BIOL, NEW BRUNSWICK, NJ 08903 USA

来源：

JOURNAL OF MICROSCOPY | 1979年 / 116卷 / JUL期

关键词：

D O I：

10.1111/j.1365-2818.1979.tb00212.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method is described for processing multiple cross‐sections from early chick embryos for scanning electron microscopy. Embryos are cut through the desired regions. Sections are affixed to a coverslip with Duco cement and critical point dried by Freon 13 or liquid CO2. This method provides a reliable means for preparing multiple cross‐sections from single embryos and eliminates the need for direct handling of brittle tissues after drying. 1979 Blackwell Science Ltd

引用

页码：287 / 290

页数：4