THE EFFECT OF CYTOKINES ON THE EXPRESSION OF MHC ANTIGENS AND ICAM-1 BY NORMAL AND TRANSFORMED SYNOVIOCYTES

被引：35

作者：

WICKS, IP

LEIZER, T

WAWRYK, SO

NOVOTNY, JR

HAMILTON, J

VITTI, G

BOYD, AW

机构：

[1] Lions Laboratory, The Walter and Eliza Hall Institute of Medical Research

[2] University of Melbourne, Department of Medicine, Royal Melbourne Hospital

来源：

AUTOIMMUNITY | 1992年 / 12卷 / 01期

基金：

英国医学研究理事会;

关键词：

CYTOKINES; MHC; ICAM;

D O I：

10.3109/08916939209146125

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We report the expression on synovial cells of cell surface molecules known to be involved in T cell activation by antigen presenting cells. Normal human synovial fibroblasts and a human synovial cell line transformed with the SV40 large T antigen were used for in vitro stimulation studies with recombinant cytokines. We demonstrate an increase in MHC-A, B, C expression in normal synovial cells in response to recombinant interferon gamma (r/IFN), tumour necrosis factor alpha and beta (rTNFα and β and interleukin-1 (rlL-lα Intercellular adhesion molecule-1 (ICAM-1) expression was increased in parallel with MHC Class I. The combination of rγlFN and rTNFa was additive in its effect on ICAM-1 expression. Northern blot analysis suggests that ICAM-1 expression in synovial cells is controlled at the level of transcription. In contrast, MHC Class II (HLA-DR) was only significantly induced by rylFN. Other stimuli including interleukin-4 (IL-4), interleukin 6 (IL-6), granulocyte macrophage colony stimulating factor (GM-CSF) and prostaglandin E2 (PGE2) did not affect the expression of ICAM-1 or MHC Class I and II. Leucocyte function antigen 3 (LFA-3) expression was not affected by any of the stimuli tested. Immunoperoxidase staining of rheumatoid synovial tissue confirmed enhanced in vivo expression of ICAM-1 in rheumatoid arthritis. These changes are discussed in the context of T cell activation in inflammatory arthritis. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：13 / 19

页数：7

共 23 条

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