ANALYSIS OF METABOLISM OF CARCINOGENIC POLYCYCLIC-HYDROCARBONS BY POSITION-SENSING PROPORTIONAL COUNTING OF THIN-LAYER CHROMATOGRAMS

被引：13

作者：

BAIRD, WM ^{[1
]}

DIAMOND, L ^{[1
]}

BORUN, TW ^{[1
]}

SHULMAN, S ^{[1
]}

机构：

[1] NAVAL RES LAB,DIV SPACE SCI,OFF NAVAL RES,WASHINGTON,DC 20375

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90058-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure which combines thin-layer chromatography with position-sensing proportional counting has been developed for analyzing the metabolism of carcinogenic polycyclic aromatic hydrocarbons. The profiles of the metabolites of [3H]benzo(a)pyrene and 7,12-[12-14C]dimethylbenz(a)anthracene produced in cell culture were comparable when obtained by this procedure and by standard methods. However, position-sensing proportional counting allows simultaneous counting of all components of a sample within 10-20 min, and thereby permits the analysis of many hydrocarbon samples in a short time. In addition, the procedure eliminates the necessity of cutting or scraping carcinogen-containing thin-layer chromatograms. © 1979.

引用

页码：165 / 169

页数：5

共 22 条

[1] BAIRD WM, 1978, CANCER RES, V38, P3432
[2] EFFECT OF 7,8-BENZOFLAVONE ON FORMATION OF BENZO[ALPHA-]PYRENE-DNA-BOUND PRODUCTS IN HAMSTER EMBRYO CELLS
BAIRD, WM
DIAMOND, L
[J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1976, 13 (01) : 67 - 75
[3] BAIRD WM, 1977, CANCER RES, V37, P3190
[4] BORUN TW, UNPUBLISHED
[5] COHEN GM, 1978, CARCINOG COMPR SURV, V3, P325
[6] METABOLISM OF POLYCYCLIC-HYDROCARBONS AND ITS RELATIONSHIP TO CANCER
DEPIERRE, JW
ERNSTER, L
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 473 (3-4) : 149 - 186
[7] Diamond L., 1977, GROWTH NUTRITION MET, V3, P421
[8] DIPPLE A, 1976, CHEM CARCINOGENS, P245
[9] FREUDENTHAL R, 1976, CARCINOGENESIS COMPR
[10] AUTORADIOGRAPHY WITH A POSITION-SENSITIVE COUNTER
GABRIEL, A
BRAM, S
[J]. FEBS LETTERS, 1974, 39 (03) : 307 - 309

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