INVOLVEMENT OF FIXLJ IN THE REGULATION OF NITROGEN-FIXATION IN AZORHIZOBIUM-CAULINODANS

被引:52
作者
KAMINSKI, PA [1 ]
ELMERICH, C [1 ]
机构
[1] INST PASTEUR,DEPT BIOTECHNOL,CNRS,URA 1300,F-75724 PARIS 15,FRANCE
关键词
D O I
10.1111/j.1365-2958.1991.tb00738.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda-GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS-lambda-L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 613bp were identified as fixL and fixJ. Kanamycin cartridges were inserted into the cloned fixL and fixJ genes and recombined into the host genome. The resulting mutants were Nif- Fix-, suggesting that the two genes were required for symbiotic nitrogen fixation and for nitrogen fixation in the free-living state. Using pnifH-lacZ and pnifA-lacZ fusions, it was shown that the FixLJ products controlled the expression of nifH and nifA in bacteria grown in the free-living state.
引用
收藏
页码:665 / 673
页数:9
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