CHARACTERIZATION OF AN HSP70 GENE FROM THE HUMAN FILARIAL PARASITE, BRUGIA-MALAYI (NEMATODA)

We have previously shown that an Onchocerca volvulus cDNA clone in lambda-gt-11 designated OvG15, potentially encoding a peptide homologous to the 70-kDa heat shock protein (Hsp70), was recognized by sera of many individuals living in a zone endemic for lymphatic filariasis and most strikingly by sera from amicrofilaremic individuals including endemic normals, those with chronic symptoms and TPE patients. Few asymptomatic microfilaremics recognized the Hsp70. We have now used the insert from the OvG15 clone to isolate the homologous gene from Brugia malayi and analyze its primary structure and expression. The data presented in this communication describe a heat-inducible member of the hsp70 gene family of B. malayi which demonstrates intriguing features of tissue specific basal level expression, developmental regulation and heat inducibility.