STABLE EXPRESSION OF THE HUMAN TSH RECEPTOR IN CHO CELLS AND CHARACTERIZATION OF DIFFERENTIALLY EXPRESSING CLONES

被引：164

作者：

PERRET, J ^{[1
]}

LUDGATE, M ^{[1
]}

LIBERT, F ^{[1
]}

GERARD, C ^{[1
]}

DUMONT, JE ^{[1
]}

VASSART, G ^{[1
]}

PARMENTIER, M ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,HOP ERASME,FAC MED,SERV GENET MED,UNITE GENET MOLEC,B-1070 BRUSSELS,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90789-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human thyrotropin receptor cDNA was transfected in CHO cells and individual clones were isolated. They were tested for their response to thyrotropin, forskolin and antibodies from a patient with high levels of thyroid stimulating antibodies. Several clones were characterized extensively with respect to membrane binding of labeled thyrotropin, cAMP accumulation in response to thyrotropin and kinetics of cAMP production. Data for three representative clones are presented. Receptor number as assessed by membrane binding of labeled thyrotropin, and cAMP production, measured in a thyrotropin response bioassay, are correlated. The Kd value for the human thyrotropin receptor expressed in CHO was estimated to be 50 pM. © 1990.

引用

页码：1044 / 1050

页数：7

共 11 条

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