QUANTITATIVE-ANALYSIS OF 1-STEAROYL-2-OLEOYL-SN-GLYCERO-3-PHOSPHOSERINE BY NEGATIVE-ION FAST-ATOM BOMBARDMENT MASS-SPECTROMETRY

被引：10

作者：

CHEN, S ^{[1
]}

KIRSCHNER, G ^{[1
]}

BENEFENATI, E ^{[1
]}

机构：

[1] FIDIA RES LABS,DEPT CHEM,I-35031 ABANO TERME,ITALY

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 06期

关键词：

D O I：

10.1002/rcm.1290040609

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Negative‐ion fast‐atom bombardment mass spectrometry was evaluated as a means for quantitative analysis of individual molecular species of glycerophosphatidylserine (GPS). With 200 ng of 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphoserine (GPS di 16:0) as an internal standard, the calibration curve for 1‐stearoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoserine (GPS 18:0–18:1) was linear over a range of 50–1000 ng (signal‐to‐noise ratio >4), with a correlation coefficient of 0.998. When the curve was derived from a mixture of GPS molecular species, only a slight variation in the ion intensity ratio of GPS 18:0–18:1 to GPS di 16:0 occurred. The method provides the possibility of quantifying GPS 18:0–18:1 among GPS species in the low nanogram range. Copyright © 1990 John Wiley & Sons Limited

引用

页码：214 / 216

页数：3

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