A UNIQUE PATHWAY OF DOUBLE-STRAND BREAK REPAIR OPERATES IN TANDEMLY REPEATED GENES

被引:150
作者
OZENBERGER, BA [1 ]
ROEDER, GS [1 ]
机构
[1] YALE UNIV,DEPT BIOL,POB 6666,NEW HAVEN,CT 06511
关键词
D O I
10.1128/MCB.11.3.1222
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The RAD52 gene product of the yeast Saccharomyces cerevisiae is required for most spontaneous recombination and almost all double-strand break (DSB) repair. In contrast to recombination elsewhere in the genome, recombination in the ribosomal DNA (rDNA) array is RAD52 independent. To determine the fate of a DSB in the rDNA gene array, a cut site for the HO endonuclease was inserted into the rDNA in a strain containing an inducible HO gene. DSBs were efficiently repaired at this site, even in the absence of the RAD52 gene product. Efficient RAD52-independent DSB repair was also observed at another tandem gene array, CUP1, consisting of 18 repeat units. However, in a smaller CUP1 array, consisting of only three units, most DSBs (ca. 80%) were not repaired and resulted in cell death. All RAD52-independent DSB repair events examined resulted in the loss of one or more repeat units. We propose a model for DSB repair in repeated sequences involving the generation of single-stranded tails followed by reannealing.
引用
收藏
页码:1222 / 1231
页数:10
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