AN IMMUNOBLOTTING PROCEDURE FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUKEMIA-VIRUS IN CATTLE

被引：14

作者：

GROVER, YP ^{[1
]}

GUILLEMAIN, B ^{[1
]}

机构：

[1] INSERM,U117,F-33076 BORDEAUX,FRANCE

来源：

JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 1992年 / 39卷 / 01期

关键词：

D O I：

10.1111/j.1439-0450.1992.tb01136.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A sensitive protein immunoblotting (Western blot) procedure has been developed for detecting anti-BLV antibodies in cattle sera. The antibodies against most of the major viral proteins could be detected. This procedure does not give any non-specific background staining and there is absence of any erroneous results due to utilisation of purified viral preparations. The procedure has been applied for detection of antibodies to BLV in a set of 74 sera samples and it has been compared with other commonly used serological tests like ELISA and agar gel immunodiffusion test.

引用

页码：48 / 52

页数：5

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