ENHANCEMENT OF GAMMA-GLUTAMYLCYSTEINE SYNTHETASE MESSENGER-RNA IN RAT-KIDNEY BY METHYL MERCURY

被引：72

作者：

WOODS, JS ^{[1
]}

DAVIS, HA ^{[1
]}

BAER, RP ^{[1
]}

机构：

[1] UNIV WASHINGTON,SCH PUBL HLTH & COMMUNITY MED,DEPT ENVIRONM HLTH,SEATTLE,WA 98195

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1992年 / 296卷 / 01期

关键词：

D O I：

10.1016/0003-9861(92)90583-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutathione (GSH), a major cellular antioxidant, is elevated 2- to 3-fold in kidneys of rats during prolonged treatment with mercury as methyl mercury hydroxide (MMH). Increased renal GSH is accompanied by a dose-and time-related elevation in the relative abundance of mRNA hybridizable to a cDNA probe which encodes renal γ-glutamylcysteine synthetase (GCS), the rate-limiting enzyme in GSH synthesis. Renal GCS mRNA is maximally elevated 4.4-fold at 3 weeks following initiation of MMH treatment. Enhancement of GSH and GCS mRNA content corresponds to a relative sparing of renal cells from oxidative tissue damage during MMH exposure. These observations suggest that increased synthesis of GSH at the genetic level occurs as an initial adaptive response to mercury-induced oxidative stress in kidney cells. © 1992.

引用

页码：350 / 353

页数：4

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