QUANTITATION OF VITAMIN-K IN HUMAN-MILK

被引:27
作者
CANFIELD, LM
HOPKINSON, JM
LIMA, AF
MARTIN, GS
SUGIMOTO, K
BURR, J
CLARK, L
MCGEE, DL
机构
[1] UNIV ARIZONA,DEPT FAMILY & COMMUNITY MED,TUCSON,AZ 85721
[2] BAYLOR UNIV,DEPT PEDIAT,HOUSTON,TX 77030
关键词
D O I
10.1007/BF02537985
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A quantitative method was developed for the assay of vitamin K in human colostrum and milk. The procedure combines preparative and analytical chromatography on silica gel in a nitrogen atmosphere followed by reversed phase high performance liquid chromatography (HPLC). Two HPLC steps were used: gradient separation with ultraviolet (UV) detection followed by isocratic separation detected electrochemically. Due to co-migrating impurities, UV detection alone is insufficient for identification of vitamin K. Exogenous vitamin K was shown to equilibrate with endogenous vitamin K in the samples. A statistical method was incorporated to control for experimental variability. Vitamin K1 was analyzed in 16 pooled milk samples from 7 donors and in individual samples from 15 donors at 1 month post-partrum. Vitamin K1 was present at 2.94±1.94 and 3.15±2.87 ng/mL in pools and in individuals, respectively. Menaquinones, the bacterial form of the vitamin, were not detected. The significance of experimental variation to studies of vitamin K in individuals is discussed. © 1990 American Oil Chemists' Society.
引用
收藏
页码:406 / 411
页数:6
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