MOLECULAR-CLONING OF THE GENES FOR ANTHRANILATE SYNTHETASE FROM STREPTOMYCES-VENEZUELAE ISP-5230

被引：6

作者：

PARADKAR, AS ^{[1
]}

STUTTARD, C ^{[1
]}

VINING, LC ^{[1
]}

机构：

[1] DALHOUSIE UNIV,DEPT MICROBIOL,HALIFAX B3H 4J1,NS,CANADA

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 78卷 / 2-3期

关键词：

TRYPTOPHAN AUXOTROPH; GENE CLONING; STREPTOMYCES; ANTHRANILATE SYNTHETASE GENE; MUTANT COMPLEMENTATION;

D O I：

10.1016/0378-1097(91)90154-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fragments of genomic DNA from Streptomyces venezuelae ISP5230 were cloned in the Escherichia coli expression vector pTZ18R and the plasmids were used to transform E. coli JA194 (trpE). The transformants included a prototrophic strain containing a recombinant plasmid, pDQ181, with an approximately 6.8-kb insert. Subcloning located the trpE-complementing DNA in a 2.4-kb segment. Transformation of E. coli ED23 (lacking both trpE and trpG functions) with plasmids containing the 2.4-kb DNA segment gave prototrophic strains exhibiting both the ASI and ASII activities of anthranilate synthetase. The results indicated that trpE and trpG are clustered in S. venezuelae. Regions hybridizing to the pDQ181 insert were present in the genomic DNA of other streptomycetes.

引用

页码：177 / 182

页数：6

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