QUANTITATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF C-14-LABELED PHOTOSYNTHETIC INTERMEDIATES IN ISOLATED INTACT CHLOROPLASTS

被引：29

作者：

GIERSCH, C

机构：

[1] Botanisches Institut der Universität Düsseldorf, D-4000 Düsseldorf

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 172卷 / APR期

关键词：

D O I：

10.1016/S0021-9673(00)90953-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid quantitative separation of photosynthetic intermediates in spinach chloroplasts is obtained on chemically modified, totally porous, silica gel resins (10 μm). Sugars are separated on a polar resin (LiChrosorb NH2) by a weakly polar eluent (acetonitrile-water), while phosphorylated compounds are eluted by a phosphate gradient on a strongly basic anion exchanger (LiChrosorb AN). The separation of phosphate esters is complete in ca. 1 h. Activity of labelled compounds is monitored on-line by means of a solid scintillator and a multi-channel analyzer. © 1979.

引用

页码：153 / 161

页数：9

共 17 条

[1] PHOSPHORUS COMPOUNDS IN HUMAN ERYTHROCYTE [J].